Usefulness of adult bovine serum for Helicobacter pylori culture media.
نویسندگان
چکیده
Fetal bovine serum (FBS) and adult bovine serum (BS) exhibited bactericidal activity against Helicobacter pylori at various levels, which were higher in BS than in FBS. The bactericidal activity was inactivated by heat treatment at 56 degrees C for 30 min. Our results demonstrated that heat-treated BS is a useful serum source of H. pylori culture medium.
منابع مشابه
The Comparative Study of three methods of microscopic, urease test and culture in diagnostic of Helicobacter pylori causing peptic ulcer
Abstract Background and objectives: Helicobacter pylori is a helical gram negative bacterium with polar flagella, discovered by Warren and Marshall in 1983. Helicobacter pylori exist in the stomach mucus tissue of less than 20% of people under 30 years old, but this amount would increase up to 40% and 60% in 60- year- old people. The aim of this study was to compare three methods of culture med...
متن کاملGrowth and survival of Helicobacter pylori in defined medium and susceptibility to Brij 78.
The gastrointestinal pathogen Helicobacter pylori requires supplementation with either fetal calf serum (FCS), bovine serum albumin (BSA), or (2,6-dimethyl)-beta-cyclodextrin (CD) for growth in a complex or defined medium. Because the availability of medium in which all components were chemically defined would facilitate metabolic studies of H. pylori, growth of the type strain, ATCC 43504, was...
متن کاملInteraction of Helicobacter pylori and its fatty acids with parietal cells and gastric H+/K(+)-ATPase.
Helicobacter pylori and the fatty acids produced by this organism were compared for their acid inhibitory activity in isolated parietal cells and their interaction with gastric H+/K(+)-ATPase. H pylori (intact organisms, sonicates, methanolic extracts, and extracts from culture medium) and the fatty acids cis 9,10-methyleneoctadecanoic acid and tetradecanoic acid inhibited at fairly high concen...
متن کاملUnique host iron utilization mechanisms of Helicobacter pylori revealed with iron-deficient chemically defined media.
Helicobacter pylori chronically infects the gastric mucosa, where it can be found free in mucus, attached to cells, and intracellularly. H. pylori requires iron for growth, but the sources of iron used in vivo are unclear. In previous studies, the inability to culture H. pylori without serum made it difficult to determine which host iron sources might be used by H. pylori. Using iron-deficient,...
متن کاملLipid profiles of Helicobacter pylori and Helicobacter mustelae grown in serum-supplemented and serum-free media.
Many of Helicobacter species have been found to have novel cholesteryl glucosides (CGs). To study the biosynthetic mechanism of CGs, the lipid profiles of H. pylori and H. mustelae grown in serum-supplemented and cholesterol-restricted serum-free media were investigated. In contrast to the serum-supplemented state, helicobacters had less CGs in the serum-free state; a trace amount of CGs and no...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of clinical microbiology
دوره 44 11 شماره
صفحات -
تاریخ انتشار 2006